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hybridization oven (g2545a  (Agilent technologies)


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    Structured Review

    Agilent technologies hybridization oven (g2545a
    Hybridization Oven (G2545a, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/the+hybridization+oven+(g2545a)/pmc02955634-327-13-16
    Average 90 stars, based on 1 article reviews
    hybridization oven (g2545a - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Gene Expression:

    Article Title: Circular RNA expression profiling identifies novel biomarkers in uterine leiomyoma.
    Article Snippet: The RNeasy Mini Kit (Cat#74106, QIAGEN, GmBH, Germany) was used to purify the labelled cRNA. .. Each slide was hybridized with 1.65 μg Cy3-labelled cRNA using the Gene Expression Hybridization Kit (Cat# 5188–5242, Agilent technologies, Santa Clara, CA, US) in the Hybridization Oven (Cat# G2545A, Agilent technologies, Santa Clara, CA, US) following the manufacturer's instructions. .. After 17 h of hybridization, the slides were washed in staining dishes (Cat# 121, Thermo Shandon, Waltham, MA, US) with a Gene Expression Wash Buffer Kit (Cat# 5188–5327, Agilent Technologies, Santa Clara, CA, US).

    Article Title: Local intestinal microbiota response and systemic effects of feeding black soldier fly larvae to replace soybean meal in growing pigs
    Article Snippet: .. Hybridization was performed as described in the One-Color Microarray-Based Gene Expression Analysis Low input Quick Amp Labeling protocol from Agilent in the hybridization oven (G2545A hybridization Oven Agilent Technologies). .. The hybridization temperature was 65 °C with rotation speed 10 rpm for 17 h. After 17 h the arrays were washed as described in the One-Color Microarray-Based Gene Expression Analysis Low input Quick Amp Labelling protocol from Agilent.

    Article Title: Dietary supplementation of zinc oxide modulates intestinal functionality during the post-weaning period in clinically healthy piglets.
    Article Snippet: .. Hybridization was performed as described in the OneColor Microarray-Based Gene Expression Analysis Low Input Quick Amp Labelling protocol from Agilent in the hybridization oven (G2545A hybridization Oven Agilent Technologies). .. The hybridization temperature was 65 °C with rotation speed 10 r/min for 17 h. After 17 h the arrays were washed as described in the OneColor Microarray-Based Gene Expression Analysis Low Input Quick Amp Labelling protocol from Agilent.

    Hybridization:

    Article Title: Circular RNA expression profiling identifies novel biomarkers in uterine leiomyoma.
    Article Snippet: The RNeasy Mini Kit (Cat#74106, QIAGEN, GmBH, Germany) was used to purify the labelled cRNA. .. Each slide was hybridized with 1.65 μg Cy3-labelled cRNA using the Gene Expression Hybridization Kit (Cat# 5188–5242, Agilent technologies, Santa Clara, CA, US) in the Hybridization Oven (Cat# G2545A, Agilent technologies, Santa Clara, CA, US) following the manufacturer's instructions. .. After 17 h of hybridization, the slides were washed in staining dishes (Cat# 121, Thermo Shandon, Waltham, MA, US) with a Gene Expression Wash Buffer Kit (Cat# 5188–5327, Agilent Technologies, Santa Clara, CA, US).

    Article Title: Serum miR-4530 sensitizes breast cancer to neoadjuvant chemotherapy by suppressing RUNX2
    Article Snippet: .. Each slide was hybridized with 100 ng of Cy3-labeled RNA using the miRNA Complete Labeling and Hyb Kit (Cat # 5190–0456, Agilent Technologies, Santa Clara, CA, USA) according to the manufacturer’s instructions and incubated in a hybridization oven (Cat # G2545A, Agilent Technologies) at 55°C for 20 hours with a rotation of 20 rpm. .. Microarray slides were scanned with an Agilent Microarray Scanner (Cat # G2565CA, Agilent Technologies).

    Article Title: Effect of PM2.5 on MicroRNA Expression and Function in Nasal Mucosa of Rats With Allergic Rhinitis.
    Article Snippet: Background: Particulate matter 2.5 (PM2.5) refers to particulate matter with aerodynamic equivalent diameter less than or equal to 2.5 mm, which is an important component of air pollution.. PM2.5 aggravates allergic rhinitis (AR) and promotes AR nasal mucosa inflammation.. Therefore, the influence of PM2.5 inhalation exposure on microRNA (miRNA) expression profiles and function in the nasal mucosa of AR rats was investigated.

    Article Title: Local intestinal microbiota response and systemic effects of feeding black soldier fly larvae to replace soybean meal in growing pigs
    Article Snippet: .. Hybridization was performed as described in the One-Color Microarray-Based Gene Expression Analysis Low input Quick Amp Labeling protocol from Agilent in the hybridization oven (G2545A hybridization Oven Agilent Technologies). .. The hybridization temperature was 65 °C with rotation speed 10 rpm for 17 h. After 17 h the arrays were washed as described in the One-Color Microarray-Based Gene Expression Analysis Low input Quick Amp Labelling protocol from Agilent.

    Article Title: Functional Characterization of Aspergillus nidulans ypkA, a Homologue of the Mammalian Kinase SGK
    Article Snippet: Finally, 100 µL of sample was placed down onto the microarray slide, which was mounted into the Agilent™ Microarray Hybridization Chamber Kit. .. The hybridization was carried out in an oven (Agilent G2545A Hybridization Oven) set to 65°C for 17 hours. .. After, microarray slides were washed according to Agilent’s instruction and scanned using GenePix® 4000B microarray scanner (Molecular Devices, USA).

    Article Title: Dietary supplementation of zinc oxide modulates intestinal functionality during the post-weaning period in clinically healthy piglets.
    Article Snippet: .. Hybridization was performed as described in the OneColor Microarray-Based Gene Expression Analysis Low Input Quick Amp Labelling protocol from Agilent in the hybridization oven (G2545A hybridization Oven Agilent Technologies). .. The hybridization temperature was 65 °C with rotation speed 10 r/min for 17 h. After 17 h the arrays were washed as described in the OneColor Microarray-Based Gene Expression Analysis Low Input Quick Amp Labelling protocol from Agilent.

    Article Title: Downregulation of Protein Tyrosine Phosphatase Receptor Type R Accounts for the Progression of Hirschsprung Disease
    Article Snippet: .. It was mounted on the rotor of the hybridization oven (G2545A, Agilent, Santa Clara, CA, United States) and hybridized overnight at 45°C. .. Chip cleaning and scanning: the chip was removed to Chip Slide Washer8; the cleaned chip was scanned with a chip scanner (G2565CA, Agilent) to obtain a hybrid image.

    Labeling:

    Article Title: Serum miR-4530 sensitizes breast cancer to neoadjuvant chemotherapy by suppressing RUNX2
    Article Snippet: .. Each slide was hybridized with 100 ng of Cy3-labeled RNA using the miRNA Complete Labeling and Hyb Kit (Cat # 5190–0456, Agilent Technologies, Santa Clara, CA, USA) according to the manufacturer’s instructions and incubated in a hybridization oven (Cat # G2545A, Agilent Technologies) at 55°C for 20 hours with a rotation of 20 rpm. .. Microarray slides were scanned with an Agilent Microarray Scanner (Cat # G2565CA, Agilent Technologies).

    Article Title: Local intestinal microbiota response and systemic effects of feeding black soldier fly larvae to replace soybean meal in growing pigs
    Article Snippet: .. Hybridization was performed as described in the One-Color Microarray-Based Gene Expression Analysis Low input Quick Amp Labeling protocol from Agilent in the hybridization oven (G2545A hybridization Oven Agilent Technologies). .. The hybridization temperature was 65 °C with rotation speed 10 rpm for 17 h. After 17 h the arrays were washed as described in the One-Color Microarray-Based Gene Expression Analysis Low input Quick Amp Labelling protocol from Agilent.

    Incubation:

    Article Title: Serum miR-4530 sensitizes breast cancer to neoadjuvant chemotherapy by suppressing RUNX2
    Article Snippet: .. Each slide was hybridized with 100 ng of Cy3-labeled RNA using the miRNA Complete Labeling and Hyb Kit (Cat # 5190–0456, Agilent Technologies, Santa Clara, CA, USA) according to the manufacturer’s instructions and incubated in a hybridization oven (Cat # G2545A, Agilent Technologies) at 55°C for 20 hours with a rotation of 20 rpm. .. Microarray slides were scanned with an Agilent Microarray Scanner (Cat # G2565CA, Agilent Technologies).

    Microarray:

    Article Title: Local intestinal microbiota response and systemic effects of feeding black soldier fly larvae to replace soybean meal in growing pigs
    Article Snippet: .. Hybridization was performed as described in the One-Color Microarray-Based Gene Expression Analysis Low input Quick Amp Labeling protocol from Agilent in the hybridization oven (G2545A hybridization Oven Agilent Technologies). .. The hybridization temperature was 65 °C with rotation speed 10 rpm for 17 h. After 17 h the arrays were washed as described in the One-Color Microarray-Based Gene Expression Analysis Low input Quick Amp Labelling protocol from Agilent.

    Article Title: Dietary supplementation of zinc oxide modulates intestinal functionality during the post-weaning period in clinically healthy piglets.
    Article Snippet: .. Hybridization was performed as described in the OneColor Microarray-Based Gene Expression Analysis Low Input Quick Amp Labelling protocol from Agilent in the hybridization oven (G2545A hybridization Oven Agilent Technologies). .. The hybridization temperature was 65 °C with rotation speed 10 r/min for 17 h. After 17 h the arrays were washed as described in the OneColor Microarray-Based Gene Expression Analysis Low Input Quick Amp Labelling protocol from Agilent.

    Expressing:

    Article Title: Circular RNA profile in diabetic peripheral neuropathy: analysis of coexpression networks of circular RNAs and mRNAs.
    Article Snippet: Hong-Hong Zhang‡ ,1, Yilian Zhang‡ ,1, Xixi Wang‡ ,1, Panpan Yang1, Bing-Yu Zhang1, Shufen Hu2, Guang-Yin Xu2 & Ji Hu*,1 1Department of Endocrinology, The Second Affiliated Hospital, Soochow University, Suzhou, 215004, PR China 2Center for Translational Pain Medicine, Institute of Neuroscience, Soochow University, Suzhou, 215123, PR China *Author for correspondence: Tel./Fax: +86 512 6778 4167; huji@suda.edu.cn ‡ Authors contributed equally



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